the IONA<sup>®</sup> test clinical-laboratories Technician
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Simple and robust
One PCR, one analysis, one report
Validated for use with ABI3500

Trisomy analysis and QST*Rplus v2
Screening for Down syndrome is offered routinely to thousands of women each year as part of standard antenatal care. For those women identified as being at high risk of carrying a Down syndrome foetus, chorionic villus sampling (10-12 weeks) or amniocentesis (14-18 weeks) is offered.

Standard cytogenetic techniques involving tissue culture and microscopic analysis can take up to 14 days to provide a diagnosis. Fluorescent insitu Hybridisation (FISH) using interphase cells is expensive, time consuming and unsuitable for high throughput use.

Elucigene QST*R kits use the DNA based QF-PCR technique. Individual results can be obtained within a few hours of receipt of samples. In routine use, turnaround reporting times of less than 24 hours from sample receipt are easily achievable.

Elucigene QST*Rplusv2 is a highly multiplexed, single tube assay containing a total of 22 markers. Autosomal markers are used to detect the three most common viable autosomal trisomies: trisomy 21 (Down syndrome), trisomy 18 (Edwards syndrome) and trisomy 13 (Patau syndrome). Additional markers on the sex chromosomes X and Y including a specific marker for the quantification of the number of X chromosomes, is useful in the diagnosis of sex chromosome aneuploidies. Detecting the three most common viable autosomal trisomies.

One PCR Illustration

One PCR

  • Simple set up – one tube per sample
  • Rapid – minimum hands on time, just add DNA
  • One step protocol – DNA extraction to PCR
  • Reduced risk of sample mix-up
  • Efficient – fewer consumables, reduced cost
One Analysis Illustration

One Analysis

  • Validated for use on the ABI3500 Genetic Analyzer
  • Highly informative – multiplexed 5 dye chemistry
  • No post-PCR manipulation
  • One capillary per sample
  • Cost effective – less instrument consumables
One Report Illustration

One Report

  • GeneMarker software application
  • Simple data review and analysis
  • Easy to use intuitive software
  • Informative single page report
  • No data transfer required
 

How it works

Quantitative Fluorescent-PCR
The method employed by Elucigene QST*R kits is the QF-PCR (Quantitative Fluorescence-Polymerase Chain Reaction) technique. Using PCR amplification, fluorescent dye labeled primers target highly polymorphic regions of DNA sequence, short tandem repeats (STRs), that are located on the chromosomes of interest. Each targeted STR marker is specific to the chromosome on which it is located, thus the copy number of the STR marker can be diagnostic of the copy number of the chromosome.

A normal diploid sample has the normal complement of two of each of the somatic chromosomes, thus two alleles of a chromosome specific STR are determined by the QF-PCR technique as two peaks in a 1:1 ratio. The observation of an extra STR allele as either a three peak pattern in a 1:1:1 ratio or two peak pattern in a 2:1 or 1:2 peak ratio is diagnostic of the presence of an additional sequence which in turn may represent an additional chromosome, as in the case of a trisomy.

Simplicity

Elucigene supports simple and easy to use analysis software for chromosome aneuploidy reporting. Results can be analysed through either Life Technologies

GeneMapper or SoftGenetics GeneMarker software. GeneMarker’s operation is simple, fast and accurate and now includes a customised Trisomy Analysis function. By selecting BPG (Best Practice Guidelines) settings within the Trisomy Analysis function users can quickly and accurately measure allele ratios to obtain a full patient sample report within minutes.

Ordering Information

Kit NameDescriptionPlatformCatalogue Code 

Elucigene QST*Rplusv2 Kit

QST*Rplusv2 is a highly multiplexed single tube assay. It comprises a total of 22 markers for chromosomes 13,18, 21, X and Y and will detect the most common viableautosomal trisomies and sex chromosome aneuploidies.

Genetic Analyser

AN0PLB2

More Info

Elucigene QST*R Kit

QST*R is a highly multiplexed single tube assay comprisinga total of 16 markers for the detection of the 3 most common viable autosomal trisomies (13, 18 and 21).

Genetic Analyser

AN003B2

More Info

Elucigene QST*R-PL Pregnancy Loss Kit

Detects trisomies of chromosome 13, 15, 16, 18, 21, 22, X and Y.

Genetic Analyser

AN6XYB1

More Info

Elucigene QST*R-13 Kit

QST*R-13 detects additional chromosome 13 specificmarkers to supplement QST*R and QST*Rplusv2 if required.

Genetic Analyser

AN013BX

More Info

Elucigene QST*R-18 Kit

QST*R-18 detects additional chromosome 18 specificmarkers to supplement QST*R and QST*Rplusv2 if required.

Genetic Analyser

AN018BX

More Info

Elucigene QST*R-21 Kit

QST*R-21 detects additional chromosome 21 specificmarkers to supplement QST*R and QST*Rplusv2 if required.

Genetic Analyser

AN021BX

More Info

Elucigene QST*RXYv2 Kit

QST*R-XYv2 comprises a total of 12 markers for boththe X and Y chromosomes. It can be used to detect sexchromosome aneuploidies.

Genetic Analyser

AN0XYB2

More Info

Elucigene QST*R21

Reflex testing for chromosome 21 – 7 STR markers in total.

Genetic Analyser

AN021BX

More Info

Elucigene QST*R/ QST*R-XYv2 Kit

A bundled product comprising one QST*R kit and one QST*R-XYv2 kit.

Genetic Analyser

AN3XYB2

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Elucigene kits are developed and manufactured within quality systems accredited to ISO9001:2008 and ISO13485:2003 and are validated as in vitro diagnostic devices in compliance with the European Community Directive 98/79/EC and the Canadian Medical Device Regulations (CMDR). Elucigene is a trademark of Delta Diagnostics (UK) Ltd. ARMS is a trademark of AstraZeneca UK Ltd. GeneMarker is a trademark of SoftGenetics Corporation. VIC®, PET® and NED™ are trademarks of Life Technologies Corporation.

Licensee Kits: This product is sold pursuant to an agreement with Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use only the purchased amount of the product and its components only for human in vitro diagnostics, solely for the clinical indication described in the accompanying instructions for use. For information on obtaining rights to use this product or its components, please contact This email address is being protected from spambots. You need JavaScript enabled to view it..