FEN006 - Fluorometric quantitation of nucleic acids: A comparison of Qubit™ and Ranger^® Technology.

For NGS, quantitation is used to assess DNA concentration of libraries prior to loading onto a flow cell, chip, or other sequencing module. This measurement is used in the calculation of library molarity, which is important for normalization in an effort to maximize usable data output. Beyond quantitation, knowledge of a library’s fragment length distribution is fundamental to molarity calculations.